Isolation of discrete repetitive sequence classes from Xenopus DNA by high temperature reassociation
نویسندگان
چکیده
منابع مشابه
Genotyping of Candida albicans isolated from animals using 25S ribosomal DNA and ALT repeats polymorphism in repetitive sequence
Background and Purpose: Candida albicans is the most prevalent Candida species isolated from animals. Candidiasis can be systemic in animals or may affect a single organ, such as the mouth, urinary tract, and skin. The aim of the present study was to determine the genetic diversity of C. albicans isolated from different animals and investigate the presence of a relationship between host specifi...
متن کاملSequence organization and developmentally regulated transcription of a family of repetitive DNA sequences of Xenopus laevis.
Members of a family of DNA sequences of Xenopus laevis have been cloned and sequenced. Molecular analyses revealed that these sequences are moderately repetitive and dispersed throughout the genome. The sequences of seven clones were compared. Two of the clones lie in the globin gene cluster; one 5' to the adult alpha 1 gene, and the other in the first intron of the tadpole alpha 1 gene. In all...
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A phenol aqueous emulsion allows the reassociation of DNA a t temperatures from 6 to 68 "C. This phenol emulsion reassociation technique (PERT) also promotes the very rapid reassociation of DNA. E . coli and human DNAs a t a concentration of 4 pg/mL reacted a t room temperature with the P E R T reassociate many thousand times faster than under the standard conditions of 0.18 M Na+, 60 O C . Sol...
متن کاملReduced-stringency DNA reassociation: sequence specific duplex formation.
Reduced-stringency DNA reassociation conditions allow low stability duplexes to be detected in prokaryotic, plant, fish, avian, mammalian, and primate genomes. Highly diverged families of sequences can be detected in avian, mouse, and human unique sequence dNAs. Such a family has been described among twelve species of birds; based on species specific melting profiles and fractionation of sequen...
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در طی سالهای اخیراستفاده ازمنابع انرژی تجدید پذیر در شبکه های مدرن بنا به دلایل زیست محیطی و اقتصادی به طور گسترده استفاده شده است همچون نیروگاههای بادی و خورشیدی .ولتاژتولیدی این نیروگاهها اغلب به فرم dc می باشد وادوات الکترونیک قدرت به عنوان مبدل و پل بین شکل موج dc وac استفاده می شوند.این پروسه باعث ایجاد پالسهایی برروی شکل موج خروجی می شود که می تواند وارد تجهیزات قدرت همچون ترانسفورماتور ی...
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ژورنال
عنوان ژورنال: Nucleic Acids Research
سال: 1978
ISSN: 0305-1048,1362-4962
DOI: 10.1093/nar/5.11.4283